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| 利用SSR分子标记鉴定“中丝2号”丝瓜 杂交种纯度 |
| Seed Purity Test for Luffa Hybrid 'Zhongsi No.2' by SSR Molecular Markers |
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| DOI： |
| 中文关键词: 关键词：丝瓜 中丝2号 纯度 SSR |
| 英文关键词:Keywords: luffa Zhongsi No.2 purity SSR |
| 基金项目:基金项目：中国农业科学院科技创新工程（34-IUA-03）；四川省科技厅应用基础研究项目（2021YJ0307）。 |
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| 中文摘要: |
| 摘要：为了建立一套丝瓜种子纯度的快速鉴定方法，采用SSR分子标记对丝瓜杂交一代品种“中丝2 号”的纯度进行鉴定，利用24对SSR引物对“中丝2号”丝瓜及其亲本的DNA进行扩增。结果表明：从24 对引物中筛选出1对特异性引物ZS-SSR24，能够分别在“中丝2号”母本和父本中扩增出特异的2条多态性 条带，大小分别为220 bp和250 bp，F1继承双亲的特异性条带，利用此标记能够鉴定出混在F1中的父本或 母本。经田间鉴定，供试丝瓜种子纯度为91.3%，这与分子标记的结果完全吻合，故该标记能够用于“中 丝2号”丝瓜品种的纯度鉴定。 |
| 英文摘要: |
| Abstract: In order to establish a rapid identification method for seed purity of luffa, SSR molecular markers were used to identify the purity of luffa hybrid 'Zhongsi No.2', and 24 pairs of SSR primers were used to amplify the DNA of luffa 'Zhongsi No.2' and its parents. The results showed that a pair of specific primers ZS-SSR24 was selected from 24 pairs of primers, and two specific polymorphic bands were amplified from the female and male parents of 'Zhongsi No.2', the size of which were 220 bp and 250 bp, respectively. F1 inherited the specific bands of parents, and the male or female parents mixed in F1 could be identified by using this marker. Field identification showed that the seed purity of the tested luffa was 91.3%, which was completely consistent with the molecular
marker results. Therefore, the marker could be used for the purity identification of 'Zhongsi No.2' luffa cultivar. |
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